The Aquarium of Rhodes is a research centre, aquarium and museum in Rhodes, Greece. It was built in the 1930s, when the island was under the Italian rule and is currently administered by the National Centre of Marine Research.

The aquarium is in the basement of the building, and is a corridor designed to look like an underwater cave. The tanks along each side of the corridor are filled with approximately 80,000 litres (21,000 US gal) of filtered seawater, providing a natural environment for the residents. The displays include sea anemones and plants, octopuses, sea urchins, corals, bivalves, crabs, lobsters, sea turtles, dolphins, seals, mollusks, sharks, and many other fish. There is a separate holding facility for incoming specimens and organisms that need special handling, where the facility also cares for sea turtles and seals that have been injured nearby.